Acid shock of Listeria monocytogenes at low environmental temperatures induces prfA, epithelial cell invasion, and lethality towards Caenorhabditis elegans
نویسندگان
چکیده
منابع مشابه
Constitutive Activation of PrfA Tilts the Balance of Listeria monocytogenes Fitness Towards Life within the Host versus Environmental Survival
PrfA is a key regulator of Listeria monocytogenes pathogenesis and induces the expression of multiple virulence factors within the infected host. PrfA is post-translationally regulated such that the protein becomes activated upon bacterial entry into the cell cytosol. The signal that triggers PrfA activation remains unknown, however mutations have been identified (prfA* mutations) that lock the...
متن کاملIrradiation inactivation of Listeria monocytogenes in low-fat ground pork at freezing and refrigeration temperatures.
Gamma radiation effectively controls Listeria monocytogenes in uncooked and in ready-to-eat foods. This study was conducted to determine if gamma radiation could be used to control L. monocytogenes in ground pork. Ground pork was contaminated with L. monocytogenes, kept at refrigeration (4 degrees C), chilling (0 degrees C), and freezing (-18 degrees C) temperatures overnight, exposed to gamma ...
متن کاملListeria monocytogenes B Modulates PrfA-Mediated Virulence Factor Expression †
Listeria monocytogenes B and positive regulatory factor A (PrfA) are pleiotropic transcriptional regulators that coregulate a subset of virulence genes. A positive regulatory role for B in prfA transcription has been well established; therefore, observations of increased virulence gene expression and hemolytic activity in a sigB strain initially appeared paradoxical. To test the hypothesis that...
متن کاملAttenuating Listeria monocytogenes Virulence by Targeting the Regulatory Protein PrfA
The transcriptional activator PrfA, a member of the Crp/Fnr family, controls the expression of some key virulence factors necessary for infection by the human bacterial pathogen Listeria monocytogenes. Phenotypic screening identified ring-fused 2-pyridone molecules that at low micromolar concentrations attenuate L. monocytogenes cellular uptake by reducing the expression of virulence genes. The...
متن کاملSigma B contributes to PrfA-mediated virulence in Listeria monocytogenes.
Transcription of the Listeria monocytogenes positive regulatory factor A protein (PrfA) is initiated from either of two promoters immediately upstream of prfA (prfAp(1) and prfAp(2)) or from the upstream plcA promoter. We demonstrate that prfAp(2) is a functional sigma(B)-dependent promoter and that a sigB deletion mutation affects the virulence phenotype of L. monocytogenes. Thus, the alternat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Genomics
سال: 2013
ISSN: 1471-2164
DOI: 10.1186/1471-2164-14-285